Structure and genetics of the O-antigen of Enterobacter cloacae C6285 containing di-N-acetyllegionaminic acid

Andrei V Filatov; Min Wang; Wenjing Wang; Andrei V Perepelov; Alexander S Shashkov; Lei Wang; Yuriy A Knirel

doi:10.1016/j.carres.2014.01.012

Structure and genetics of the O-antigen of Enterobacter cloacae C6285 containing di-N-acetyllegionaminic acid

Carbohydr Res. 2014 Jun 17:392:21-4. doi: 10.1016/j.carres.2014.01.012. Epub 2014 Jan 24.

Authors

Andrei V Filatov¹, Min Wang², Wenjing Wang³, Andrei V Perepelov⁴, Alexander S Shashkov¹, Lei Wang², Yuriy A Knirel¹

Affiliations

¹ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
² TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, PR China; Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, TEDA, Tianjin 300457, PR China.
³ Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, PR China.
⁴ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia. Electronic address: perepel@ioc.ac.ru.

PMID: 24837902
DOI: 10.1016/j.carres.2014.01.012

Abstract

On mild acid degradation of the lipopolysaccharide of Enterobacter cloacae C6285, the O-polysaccharide was cleaved at residues of 5,7-diacetamido-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic acid (di-N-acetyllegionaminic acid, Leg5Ac7Ac) in the main chain. The resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with (1)H and (13)C NMR spectroscopy, and the following structure of the tetrasaccharide repeating unit of the linear O-polysaccharide was established: →4)-α-d-Galp-(1→4)-α-Legp5Ac7Ac-(2→3)-β-d-Galp-(1→3)-β-d-GalpNAc-(1→ The O-antigen gene cluster of E. cloacae C6285 was sequenced, the gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.

Keywords: Bacterial polysaccharide structure; Enterobacter cloacae; Legionaminic acid; O-Antigen; O-Antigen gene cluster.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Enterobacter cloacae / genetics*
Molecular Sequence Data
Multigene Family
O Antigens / chemistry*
O Antigens / genetics*
Sialic Acids / chemistry*

Substances

5,7-diacetamido-8-O-acetyl-3,5,7,9-tetradeoxy-glycero-talo-nonulosonic acid
O Antigens
Sialic Acids