Genetic variations of mitochondrial antiviral signaling gene (MAVS) in domestic chickens

Dandan Yu; Ling Xu; Li Peng; Shi-Yi Chen; Yi-Ping Liu; Yong-Gang Yao

doi:10.1016/j.gene.2014.05.029

Genetic variations of mitochondrial antiviral signaling gene (MAVS) in domestic chickens

Gene. 2014 Jul 25;545(2):226-32. doi: 10.1016/j.gene.2014.05.029. Epub 2014 May 14.

Authors

Dandan Yu¹, Ling Xu², Li Peng², Shi-Yi Chen³, Yi-Ping Liu⁴, Yong-Gang Yao⁵

Affiliations

¹ Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China.
² Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China; Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China.
³ Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, China.
⁴ Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, China; College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan 625014, China.
⁵ Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China. Electronic address: ygyaozh@gmail.com.

PMID: 24835312
DOI: 10.1016/j.gene.2014.05.029

Abstract

Mitochondrial antiviral signaling (MAVS) gene plays a key role in antiviral regulation in mammals potentially by activating IRF3/7 and NF-κB and leading to the induction of type I interferon (IFN)-mediated antiviral and inflammatory responses. In this study, we screened genetic polymorphisms of the MAVS gene in various Chinese domestic chicken breeds/populations and evaluated its potential effect on gene expression. Among the sequenced fragment (4678bp), a total of 75 single nucleotide polymorphisms (SNPs) were identified in 46 chickens from 10 breeds/populations, including 30 coding SNPs and 45 non-coding SNPs. Extremely high haplotype diversity (37 nucleotide haplotypes, 18 amino acid haplotypes) was observed in the coding region (CDS), and a similar pattern of high polymorphisms was also observed for the 3'-untranslated region (3'-UTR). Luciferase assays of two representative 3'-UTR haplotypes were performed in both HEK293 cells and DF-1 chicken fibroblast cells, and we found that they were differentially associated with different abilities on regulating mRNA expression level (P<0.05). Collectively, we observed a considerably high genetic variability of the MAVS gene, and the 3'-UTR variants had an ability to regulate mRNA expression. These results would cast some clues on understanding the potential role of MAVS on viral resistance in chicken.

Keywords: 3'-UTR; Chicken; Haplotype; MAVS; Polymorphism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Chickens / classification
Chickens / genetics*
Gene Expression
Genes, Mitochondrial*
Genes, Reporter
Genetic Variation*
Haplotypes
Humans
Molecular Sequence Data
Nucleic Acid Conformation
Phylogeny
Polymorphism, Single Nucleotide

Substances

3' Untranslated Regions