HepG2.2.15 as a model for studying cell protrusion and migration regulated by S100 proteins

Shiying Liu; Rui Zhou; Jian Zhong; Chunlai Nie; Zhu Yuan; Liangxue Zhou; Na Luo; Chunting Wang; Aiping Tong

doi:10.1016/j.bbrc.2014.05.010

HepG2.2.15 as a model for studying cell protrusion and migration regulated by S100 proteins

Biochem Biophys Res Commun. 2014 Jun 20;449(1):175-81. doi: 10.1016/j.bbrc.2014.05.010. Epub 2014 May 14.

Authors

Shiying Liu¹, Rui Zhou¹, Jian Zhong¹, Chunlai Nie¹, Zhu Yuan¹, Liangxue Zhou², Na Luo³, Chunting Wang⁴, Aiping Tong⁵

Affiliations

¹ State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China.
² Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, PR China.
³ Nankai University School of Medicine, Tianjin, PR China.
⁴ State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China. Electronic address: chtwang@163.com.
⁵ State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China. Electronic address: aipingtong@scu.edu.cn.

PMID: 24832736
DOI: 10.1016/j.bbrc.2014.05.010

Abstract

Much of the difficulty in elucidating the precise function of S100 protein family has been attributed to functional redundancy and compensation by its conserved family members. In this study, we showed that seven S100 family members were almost totally undetectable in HepG2.2.15 cells, while all of them were highly expressed in its parental HepG2 cells. Re-expression of S100 proteins in HepG2.2.15 cells can partially rescue their defects in cell protrusion and migration through the regulation of cytoskeletons and adhesions. Thus, HepG2.2.15 can serve as a useful model for studying cell protrusion and migration regulated by S100 proteins.

Keywords: Cell migration; Cell protrusion; Cytoskeleton; Hepg2.2.15 cells; S100 proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Enlargement*
Cell Movement / physiology*
Hep G2 Cells / classification
Hep G2 Cells / pathology*
Hep G2 Cells / physiology*
Humans
S100 Proteins / physiology*

Substances

S100 Proteins