Multiplex PCR assay for identification of six different Staphylococcus spp. and simultaneous detection of methicillin and mupirocin resistance

J Clin Microbiol. 2014 Jul;52(7):2698-701. doi: 10.1128/JCM.00918-14. Epub 2014 May 14.

Abstract

We describe a new, efficient, sensitive, and fast single-tube multiple-PCR protocol for the identification of the most clinically significant Staphylococcus spp. and the simultaneous detection of the methicillin and mupirocin resistance loci. The protocol identifies at the species level isolates belonging to S. aureus, S. epidermidis, S. haemolyticus, S. hominis, S. lugdunensis, and S. saprophyticus.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Drug Resistance, Bacterial*
  • Humans
  • Methicillin / pharmacology
  • Molecular Diagnostic Techniques
  • Multiplex Polymerase Chain Reaction / methods*
  • Mupirocin / pharmacology
  • Sensitivity and Specificity
  • Staphylococcal Infections / diagnosis*
  • Staphylococcal Infections / microbiology*
  • Staphylococcus / classification*
  • Staphylococcus / drug effects*
  • Staphylococcus / isolation & purification
  • Time Factors

Substances

  • Anti-Bacterial Agents
  • Mupirocin
  • Methicillin