In vitro culture for doubled haploids: tools for molecular breeding

Sue Broughton; Parminder K Sidhu; Philip A Davies

doi:10.1007/978-1-4939-0446-4_14

In vitro culture for doubled haploids: tools for molecular breeding

Methods Mol Biol. 2014:1145:167-89. doi: 10.1007/978-1-4939-0446-4_14.

Authors

Sue Broughton¹, Parminder K Sidhu, Philip A Davies

Affiliation

¹ Department of Agriculture and Food, Government of WA, 3 Baron-Hay Court, South Perth, 6151, WA, Australia, sue.broughton@agric.wa.gov.au.

PMID: 24816668
DOI: 10.1007/978-1-4939-0446-4_14

Abstract

Doubled haploid (DH) techniques using immature microspores or embryos rescued in vitro following interspecific hybridization are important tools for breeding wheat and barley and for generating populations for gene mapping purposes. Here, we describe methods being used for the successful production of spring wheat and barley DHs in Australia.

MeSH terms

Breeding / methods*
Chromosomes, Plant
Culture Techniques
DNA Shuffling
Flowers / cytology
Flowers / genetics
Flowers / growth & development
Haploidy
Hordeum / cytology
Hordeum / genetics
Hordeum / growth & development
Plant Roots / cytology
Plant Roots / genetics
Plant Roots / growth & development
Seedlings / cytology
Seedlings / genetics
Seedlings / growth & development
Seeds / cytology
Seeds / genetics
Seeds / growth & development
Triticum / cytology
Triticum / genetics
Triticum / growth & development