Developmental validation of the EX20+4 system

Shuanglin Li; Chao Liu; Hong Liu; Jianye Ge; Bruce Budowle; Changhui Liu; Weiguo Zheng; Fayuan Li; Binwen Ge

doi:10.1016/j.fsigen.2014.03.001

Developmental validation of the EX20+4 system

Forensic Sci Int Genet. 2014 Jul:11:207-13. doi: 10.1016/j.fsigen.2014.03.001. Epub 2014 Mar 19.

Authors

Shuanglin Li¹, Chao Liu², Hong Liu³, Jianye Ge⁴, Bruce Budowle⁵, Changhui Liu⁶, Weiguo Zheng⁷, Fayuan Li⁸, Binwen Ge⁹

Affiliations

¹ Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou 510030, China; Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510089, China. Electronic address: uzhiyanzhai@126.com.
² Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou 510030, China; Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510089, China. Electronic address: Liuchaogaj@21cn.com.
³ Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou 510030, China. Electronic address: 79liuhong@163.com.
⁴ Institute of Applied Genetics, Department of Forensic and Investigative Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USA. Electronic address: gejianye@gmail.com.
⁵ Institute of Applied Genetics, Department of Forensic and Investigative Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USA; Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: Bruce.Budowle@unthsc.edu.
⁶ Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou 510030, China. Electronic address: liuchanghui2007@gmail.com.
⁷ AGCU ScienTch Incorporation, Wuxi 214174, China. Electronic address: zhengweiguo@agcu.cn.
⁸ AGCU ScienTch Incorporation, Wuxi 214174, China. Electronic address: lifayuan2009@163.com.
⁹ AGCU ScienTch Incorporation, Wuxi 214174, China. Electronic address: gebingwen@AGCU.com.

PMID: 24815370
DOI: 10.1016/j.fsigen.2014.03.001

Abstract

The EX20+4Y System is a polymerase chain reaction (PCR)-based amplification kit that enables typing of 19 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, TH01, TPOX, vWA, Penta D, Penta E, D2S1338, D19S433, D12S391, D6S1043), four widely used Y chromosome-specific STR (Y-STR) loci (DYS458, DYS456, DYS391, DYS635), and amelogenin. In this study, this multiplex system was validated for sensitivity of detection, DNA mixtures, inhibitor tolerance, species specificity based on the Scientific Working Group on DNA Analysis methods (SWGDAM) developmental validation guidelines, and the Chinese criteria for the human fluorescent STR multiplex PCR reagent. The results show that the EX20+4 System is a robust and reliable amplification kit which can be used for human identification testing.

Keywords: DNA typing; Developmental validation; Forensic science; Multiplexing; Short tandem repeat (STR).

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Chromosomes, Human, Y*
DNA / genetics
Electrophoresis, Capillary
Female
Humans
Male
Microsatellite Repeats / genetics
Polymerase Chain Reaction
Reproducibility of Results

Substances

DNA