Pyranocoumarins from Glehnia littoralis inhibit the LPS-induced NO production in macrophage RAW 264.7 cells

Bioorg Med Chem Lett. 2014 Jun 15;24(12):2717-9. doi: 10.1016/j.bmcl.2014.04.046. Epub 2014 Apr 21.

Abstract

A new dihydropyranocoumarin, (+)-cis-(3'S,4'S)-diisobutyrylkhellactone (1), together with five known compounds, 3'-senecioyl-4'-acetylkhellactone (2), 3'-isovaleryl-4'-acetylkhellactone (3), 3',4'-disenecioylkhellactone (4), 3'-isovaleryl-4'-senecioylkhellactone (5), and 3',4'-diisovalerylkhellactone (6), was isolated from Glehnia littoralis. Their chemical structures were elucidated based on the spectroscopic data interpretation, particularly 1D and 2D NMR data including HMQC and HMBC. All the isolated compounds showed the potential to inhibit LPS-induced nitric oxide production in RAW 264.7 cells with IC50 values ranging from 7.4 to 44.3μM.

Keywords: Apiaceae; Dihydropyranocoumarin; Glehnia littoralis; Nitric oxide production inhibitor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apiaceae / chemistry*
  • Cell Line
  • Inhibitory Concentration 50
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects*
  • Mice
  • Molecular Structure
  • Nitric Oxide / antagonists & inhibitors*
  • Nitric Oxide / metabolism
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Pyranocoumarins / chemistry*
  • Pyranocoumarins / isolation & purification
  • Pyranocoumarins / pharmacology*

Substances

  • Lipopolysaccharides
  • Plant Extracts
  • Pyranocoumarins
  • Nitric Oxide