Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

Kunio Hirata; Kyoko Shinzawa-Itoh; Naomine Yano; Shuhei Takemura; Koji Kato; Miki Hatanaka; Kazumasa Muramoto; Takako Kawahara; Tomitake Tsukihara; Eiki Yamashita; Kensuke Tono; Go Ueno; Takaaki Hikima; Hironori Murakami; Yuichi Inubushi; Makina Yabashi; Tetsuya Ishikawa; Masaki Yamamoto; Takashi Ogura; Hiroshi Sugimoto; Jian-Ren Shen; Shinya Yoshikawa; Hideo Ago

doi:10.1038/nmeth.2962

Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

Nat Methods. 2014 Jul;11(7):734-6. doi: 10.1038/nmeth.2962. Epub 2014 May 11.

Authors

Kunio Hirata¹, Kyoko Shinzawa-Itoh², Naomine Yano³, Shuhei Takemura⁴, Koji Kato², Miki Hatanaka⁴, Kazumasa Muramoto⁴, Takako Kawahara⁴, Tomitake Tsukihara⁵, Eiki Yamashita⁶, Kensuke Tono⁷, Go Ueno⁸, Takaaki Hikima⁸, Hironori Murakami⁸, Yuichi Inubushi⁸, Makina Yabashi⁸, Tetsuya Ishikawa⁸, Masaki Yamamoto⁸, Takashi Ogura⁹, Hiroshi Sugimoto⁸, Jian-Ren Shen¹⁰, Shinya Yoshikawa⁴, Hideo Ago⁸

Affiliations

¹ 1] RIKEN SPring-8 Center, Sayo, Japan. [2] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [3].
² 1] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [2].
³ 1] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [2] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
⁴ Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
⁵ 1] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [2] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [3] Institute for Protein Research, Osaka University, Suita, Japan.
⁶ Institute for Protein Research, Osaka University, Suita, Japan.
⁷ Japan Synchrotron Radiation Research Institute, Sayo, Japan.
⁸ RIKEN SPring-8 Center, Sayo, Japan.
⁹ Picobiology Institute, Graduate School of Life Science, University of Hyogo, Sayo, Japan.
¹⁰ Photosynthesis Research Center, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.

PMID: 24813624
DOI: 10.1038/nmeth.2962

Abstract

We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Crystallography / methods*
Electron Transport Complex IV / chemistry*
Electron Transport Complex IV / radiation effects
Lasers*

Substances

Electron Transport Complex IV