A total of 2 769 children with allergic disease were admitted from October 2010 to December in Ningbo Women & Children's Hospital. Fourteen kinds of serum specific-IgE were detected by immunoblotting method. Among the 2 769 children, the total positive rate of sIgE was 76.0% (2 105/2 769). The sIgE positive rate in males (80.0%, 1 344/1 679) was significantly higher than that of females (69.8%, 761/1 090)(P < 0.05). The top three allergens were dust mites (33.2%), milk (12.6%) and fungi (12.6%). There were statistical differences of positive reaction to dust mites among 4 seasons (19.4%, 33.9%, 39.3%, 36.0%) (P < 0.05). Significant differences were found in the positive rates of dust mites, milk, fungi, and egg among the age groups (P < 0.05). The level of dust mite specific IgE were mainly in grade 3-6 (613/918, 66.8%).