Molecular detection of Sarcocystis species in slaughtered sheep by PCR-RFLP from south-western of Iran

Hossein Hamidinejat; Hossein Moetamedi; Alireza Alborzi; Abbas Hatami

doi:10.1007/s12639-012-0231-z

Molecular detection of Sarcocystis species in slaughtered sheep by PCR-RFLP from south-western of Iran

J Parasit Dis. 2014 Jun;38(2):233-7. doi: 10.1007/s12639-012-0231-z. Epub 2013 Jan 10.

Authors

Hossein Hamidinejat¹, Hossein Moetamedi², Alireza Alborzi¹, Abbas Hatami³

Affiliations

¹ Department of Pathobiology, Veterinary Faculty, Shahid Chamran University, Ahvaz, Iran.
² Department of Biology Science Faculty, Shahid Chamran University, Ahvaz, Iran.
³ M.Sc of Parasitology Graduated, Veterinary Faculty, Shahid Chamran University, Ahvaz, Iran.

Abstract

Sarcocystis spp. are the cyst forming protozoan parasites that prevalent in livestock all around the world. In the presented work, we examined 40 macroscopic and 40 microscopic sarcocysts from Khouzestan and Lorestan provinces, south-western Iran, utilizing PCR-RFLP based on amplification of 18S rRNA gene. Using AvaI, HindII, TaqI and EcoRI restriction enzymes the results represented Sarcocystis gigantea in both macroscopic and microscopic cysts. This result supports the importance of molecular investigations on characterization of Sarcocystis species when we aimed to assess the reliable control and preventive programs.

Keywords: 18S rRNA; Iran; PCR–RFLP; Sarcocystis spp.; Sheep.