Rapid identification of glycerophospholipids from RAW264.7 cells by UPLC/ESI -QTOF-MS

Yuqi She; Jianao Song; E Yang; Lili Zhao; Yanmei Zhong; Wen Rui; Yifan Feng; Xia Wu

doi:10.1002/bmc.3216

Rapid identification of glycerophospholipids from RAW264.7 cells by UPLC/ESI -QTOF-MS

Biomed Chromatogr. 2014 Dec;28(12):1744-55. doi: 10.1002/bmc.3216. Epub 2014 May 8.

Authors

Yuqi She¹, Jianao Song, E Yang, Lili Zhao, Yanmei Zhong, Wen Rui, Yifan Feng, Xia Wu

Affiliation

¹ Central Laboratory, Guangdong Pharmaceutical University, Guangzhou, 510006, People's Republic of China.

PMID: 24806596
DOI: 10.1002/bmc.3216

Abstract

The study aims to develop a rapid, sensitive ultra-performance liquid chromatography coupled with an electrospray ionization quadruple time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS) analytical method for identifying glycerophospholipids (GPLs) from RAW264.7 cells. A total of 78 GPLs including 22 phosphatidylethanolamines (PEs), 49 phosphatidylcholines (PCs), four phosphatidylglycerols, one phosphatidylinositol and two unknown GPLs were identified. PC (14:0/16:1), PC (14:0/16:0), PE (0:0/20:3), PE (22:5/0:0) and PE (22:3/0:0) were identified for the first time. The UPLC/Q-TOF-MS method is suitable for targeting analysis of GPLs from RAW264.7 cells, which allows us to find out new GPLs compositions related to inflammatory diseases and to explain their pharmacological roles in inflammatory process.

Keywords: RAW264.7 cell; UPLC/Q-TOF-MS; fragmentation regularity; glycerophospholipid; identification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Chromatography, High Pressure Liquid / methods*
Glycerophospholipids / analysis*
Glycerophospholipids / chemistry*
Glycerophospholipids / isolation & purification
Mice
Reproducibility of Results
Tandem Mass Spectrometry / methods*

Substances

Glycerophospholipids