Abstract
To identify the most active antimicrobial fraction of Folium Syringae, four common pathogens were used in an in vitro screening. The results indicated that the combination of the 30% and 60% ethanol fraction (FSC) obtained from the water extraction was the most active fraction with a minimal inhibitory concentration of 0.65 mg mL(-1). FSC was also found to be able to protect mice from a lethal infection of Staphylococcus aureus at clinical dosage (0.2 g kg(-1)) with a survival rate of 83.3%. The antibacterial activity of FSC was then tested using the serum pharmacology method which revealed that FSC exhibits a more long-lasting activity than the positive control (levofloxacin hydrochloride). The main components were confirmed to be iridoid glycosides and flavones by HPLC-MS analysis.
Keywords:
Folium Syringae; HPLC–MS; antibacterial activity; fraction; medicated serum.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Bacterial Agents / blood
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology*
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Flavones / blood
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Flavones / chemistry
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Flavones / isolation & purification*
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Flavones / pharmacology*
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Glycosides / chemistry
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Glycosides / isolation & purification
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Glycosides / pharmacology
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Iridoid Glycosides / blood
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Iridoid Glycosides / chemistry
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Iridoid Glycosides / isolation & purification*
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Iridoid Glycosides / pharmacology*
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Kaempferols / blood
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Kaempferols / chemistry
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Kaempferols / isolation & purification*
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Kaempferols / pharmacology*
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Luteolin / blood
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Luteolin / chemistry
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Luteolin / isolation & purification
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Luteolin / pharmacology
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Male
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Medicine, Chinese Traditional
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Mice
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Microbial Sensitivity Tests
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Molecular Structure
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Staphylococcus aureus / drug effects
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Syringa / chemistry*
Substances
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Anti-Bacterial Agents
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Flavones
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Glycosides
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Iridoid Glycosides
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Kaempferols
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syringopicroside
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Luteolin