A new spiroplasma isolate from the field cricket (Gryllus bimaculatus) in Taiwan

Yu-Shin Nai; Ping-Yi Su; Yu-Hsiang Hsu; Ching-Hao Chiang; Jae Su Kim; Yue-Wen Chen; Chung-Hsiung Wang

doi:10.1016/j.jip.2014.04.006

A new spiroplasma isolate from the field cricket (Gryllus bimaculatus) in Taiwan

J Invertebr Pathol. 2014 Jul:120:4-8. doi: 10.1016/j.jip.2014.04.006. Epub 2014 May 4.

Authors

Yu-Shin Nai¹, Ping-Yi Su², Yu-Hsiang Hsu², Ching-Hao Chiang³, Jae Su Kim⁴, Yue-Wen Chen⁵, Chung-Hsiung Wang⁶

Affiliations

¹ Institute of Zoology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC; Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 561-756, Republic of Korea.
² Institute of Zoology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC.
³ Department of Leisure Business Management, De Lin Institute of Technology, Taiwan, ROC.
⁴ Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 561-756, Republic of Korea.
⁵ Department of Biotechnology and Animal Science, National Ilan University, No. 1, Sec. 1, Shen Nung Road, Ilan 26047, Taiwan, ROC.
⁶ Institute of Zoology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC; Department of Biotechnology and Animal Science, National Ilan University, No. 1, Sec. 1, Shen Nung Road, Ilan 26047, Taiwan, ROC. Electronic address: wangch@ntu.edu.tw.

PMID: 24801581
DOI: 10.1016/j.jip.2014.04.006

Abstract

We briefly described the morphology and transmission pathway of a Spiroplasma sp. isolated from the field cricket, Gryllus bimaculatus in Taiwan, followed by the phylogenetic analysis based on the 16S rRNA gene sequence. The cricket spiroplasma infected the hemolymph, gut, muscle tissues and tracheal cells; therefore we suggest that the pathogen invaded tissues and organs from the hemolymph through the tracheal system and the endoplasmic reticular system. Based on 16S rRNA gene sequences and the phylogeny, this spiroplasma was most closely related to Spiroplasma platyhelix (Identity=95%) isolated from the dragonfly Pachydiplax longipennis and belongs to the Ixodetis clade.

Keywords: 16S rRNA; Gryllus bimaculatus; Phylogeny; Spiroplasma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA, Bacterial / genetics
Gryllidae / microbiology*
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
Spiroplasma / genetics*
Taiwan

Substances

DNA, Bacterial