Characterization of cis- and trans-octadecenoic acid positional isomers in edible fat and oil using gas chromatography-flame ionisation detector equipped with highly polar ionic liquid capillary column

Kazuaki Yoshinaga; Masaharu Asanuma; Hoyo Mizobe; Koichi Kojima; Toshiharu Nagai; Fumiaki Beppu; Naohiro Gotoh

doi:10.1016/j.foodchem.2014.03.069

Characterization of cis- and trans-octadecenoic acid positional isomers in edible fat and oil using gas chromatography-flame ionisation detector equipped with highly polar ionic liquid capillary column

Food Chem. 2014 Oct 1:160:39-45. doi: 10.1016/j.foodchem.2014.03.069. Epub 2014 Mar 21.

Authors

Kazuaki Yoshinaga¹, Masaharu Asanuma², Hoyo Mizobe¹, Koichi Kojima¹, Toshiharu Nagai¹, Fumiaki Beppu², Naohiro Gotoh³

Affiliations

¹ Tsukishima Foods Industry Co. Ltd., 3-17-9 Higashi Kasai, Edogawa-ku, Tokyo 134-8520, Japan.
² Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
³ Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan. Electronic address: ngotoh@kaiyodai.ac.jp.

PMID: 24799206
DOI: 10.1016/j.foodchem.2014.03.069

Abstract

In this study, the characterisation of all cis- and trans-octadecenoic acid (C18:1) positional isomers in partially hydrogenated vegetable oil (PHVO) and milk fat, which contain several cis- and trans-C18:1 positional isomers, was achieved by gas chromatography-flame ionisation detector equipped with a highly polar ionic liquid capillary column (SLB-IL111). Prior to analysis, the cis- and trans-C18:1 fractions in PHVO and milk fat were separated using a silver-ion cartridge. The resolution of all cis-C18:1 positional isomers was successfully accomplished at the optimal isothermal column temperature of 120 °C. Similarly, the positional isomers of trans-C18:1, except for trans-6-C18:1 and trans-7-C18:1, were separated at 120 °C. The resolution of trans-6-C18:1 and trans-7-C18:1 isomers was made possible by increasing the column temperature to 160 °C. This analytical method is suitable for determining the cis- and trans-C18:1 positional isomers in edible fats and oils.

Keywords: Gas chromatography; Hydrogenated fat; Ionic liquid; Milk fat; Trans fatty acids.

MeSH terms

Animals
Chromatography, Gas / methods*
Fats / analysis*
Flame Ionization
Ionic Liquids / chemistry
Isomerism
Milk / chemistry
Plant Oils / analysis*
Stearic Acids / analysis*

Substances

5-octadecenoic acid
Fats
Ionic Liquids
Plant Oils
Stearic Acids