Combined effect between CHRNB3-CHRNA6 region gene variant (rs6474412) and smoking in psoriasis vulgaris severity

Gene. 2014 Jul 10;544(2):123-7. doi: 10.1016/j.gene.2014.04.070. Epub 2014 Apr 30.

Abstract

Background: Many factors associated with causing psoriasis have been reported, such as the genetic and environmental factors. Smoking is one of the well-established environmental risk factors for psoriasis and also associated with the disease severity. In addition, several studies of psoriasis and psoriatic arthritis have documented gene-environment interactions involving smoking behavior. Although gene polymorphisms on nicotinic acetylcholine receptor subunits CHRNB3-CHRNA6 region gene have been found to correlate with smoking behavior and lung cancer susceptibility in Chinese Han population, the combined effect between the smoking-related genetic variants and smoking behavior on psoriasis vulgaris (PV) has been unreported.

Objective: To evaluate the combined effect of the smoking-related (rs6474412-C/T) polymorphism on CHRNB3-CHRNA6 region gene and smoking behavior on PV risk and clinic traits in Chinese Han population.

Methods: A hospital-based case-control study including 672 subjects (355 PV cases and 317 controls) was conducted. The variant of rs6474412 was typed by SNaPshot Multiplex Kit (Applied Biosystems Co., USA).

Results: The higher body mass index (BMI≥25), smoking behavior and alcohol consumption were risk factors for PV, and the estimated ORs were 1.55 (95% CI, 1.09-2.29), 1.74 (95% CI, 1.22-2.49) and 1.81 (95% CI, 1.25-2.62) respectively. The smoking patients had more severe conditions than non-smokers (OR=1.71, 95% CI, 1.08-2.70, P=0.020). The alleles and genotypes of rs6474412 were not associated with risk of PV, but the combined effect of rs6474412 genotype (TT) and smoking behavior increased severity of PV (OR=5.95; 95% CI, 1.39-25.31; P<0.05; adjusted OR=2.20; 95% CI, 1.55-3.14; P<0.001).

Conclusions: Our results demonstrate that the combined effect of rs6474412-C/T polymorphism in smoking-related CHRNB3-CHRNA6 region gene and smoking behavior may not confer risk to PV, but may have impact on PV severity in Chinese Han population.

Keywords: CHRNB3–CHRNA6 region gene variant; Combined effect; Psoriasis vulgaris; Smoking.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Alcohol Drinking / adverse effects
  • Asian People / genetics
  • Body Mass Index
  • Case-Control Studies
  • China
  • Disease Progression
  • Environmental Exposure
  • Female
  • Gene-Environment Interaction
  • Genetic Predisposition to Disease
  • Genetic Variation
  • Hospitalization
  • Humans
  • Male
  • Middle Aged
  • Polymorphism, Single Nucleotide
  • Psoriasis / etiology
  • Psoriasis / genetics*
  • Receptors, Nicotinic / genetics*
  • Risk Factors
  • Severity of Illness Index*
  • Smoking / adverse effects
  • Smoking / genetics*
  • Young Adult

Substances

  • CHRNA6 protein, human
  • CHRNB3 protein, human
  • Receptors, Nicotinic