Traditional methods for isolation of Listeria monocytogenes

Rui Magalhães; Cristina Mena; Vânia Ferreira; Gonçalo Almeida; Joana Silva; Paula Teixeira

doi:10.1007/978-1-4939-0703-8_2

Traditional methods for isolation of Listeria monocytogenes

Methods Mol Biol. 2014:1157:15-30. doi: 10.1007/978-1-4939-0703-8_2.

Authors

Rui Magalhães¹, Cristina Mena, Vânia Ferreira, Gonçalo Almeida, Joana Silva, Paula Teixeira

Affiliation

¹ CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Dr. António Bernardino Almeida, Porto, 4200-072, Portugal.

PMID: 24792545
DOI: 10.1007/978-1-4939-0703-8_2

Abstract

Conventional methods for the detection of Listeria monocytogenes in foods and environmental samples relies on selective pre-enrichment, enrichment, and plating. This is followed by confirmation of suspected colonies by testing a limited number of biochemical markers.

MeSH terms

Agar / chemistry
Bacteriological Techniques / instrumentation
Bacteriological Techniques / methods
Colony Count, Microbial
Culture Media / chemistry
Environmental Microbiology*
Food Microbiology / instrumentation
Food Microbiology / methods
Listeria monocytogenes / isolation & purification*

Substances

Culture Media
Agar