Many studies have been conducted on the treatment of burns because they are important in morbidity and mortality. These studies are mainly focused on improving care and quality of life of patients. The aim of this study is the induction of standardized full-thickness burns of the skin of rats using the CO2 laser. The results show that non-contact technique using the CO2 laser is effective to induce such standardization of burning and is an important step in determining the efficiency of different therapies used in treating burns.