Abstract
This work proposes a miniaturized system able to perform multiple cell capture followed by cell-type selective release from a biochip surface. Unlabelled lymphocytes were first specifically captured onto a DNA array by antibody-DNA conjugates. The immobilized cells were subsequently released under spatiotemporal control within local heating generated by intense Surface Plasmon Resonance (SPR) produced by laser illumination.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / chemistry*
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B-Lymphocytes / chemistry*
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B-Lymphocytes / metabolism
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Cells, Immobilized / cytology
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Cells, Immobilized / metabolism
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DNA / chemistry*
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Mice
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Surface Plasmon Resonance* / instrumentation
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Surface Plasmon Resonance* / methods
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T-Lymphocytes / cytology*
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T-Lymphocytes / metabolism
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Tissue Array Analysis* / instrumentation
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Tissue Array Analysis* / methods