Efficient gene carriers composed of 2-hydroxypropyl-β-cyclodextrin, ethanolamine-functionalized poly(glycidyl methacrylate), and poly((2-dimethyl amino)ethyl methacrylate) by combination of ATRP and click chemistry

Macromol Biosci. 2014 Aug;14(8):1135-48. doi: 10.1002/mabi.201400062. Epub 2014 May 2.

Abstract

In this work, a simple one-step method is first employed to produce the bromoisobutyryl-terminated 2-hydroxypropyl-β-cyclodextrin (HPCD-Br). The pendant epoxy groups of poly(glycidyl methacrylate) block prepared via ATRP from HPCD-Br can be reacted with ethanolamine to produce HPCD-PGEA which exhibits much lower cytotoxicity and better gene transfection yield than polyethylenimine (25 kDa) in COS7 and HepG2 cell lines. Moreover, poly((2-dimethyl amino)ethyl methacrylate) blocks can be incorporated into low-molecular-weight HPCD-PGEA via "click" reaction to further enhance the gene transfection efficiency in HepG2 cell lines.

Keywords: 2-hydroxypropyl-β-cyclodextrin; ATRP; EA; GMA; PDMAEMA; click reaction; gene delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Hydroxypropyl-beta-cyclodextrin
  • Animals
  • COS Cells
  • Cell Survival
  • Chlorocebus aethiops
  • Click Chemistry / methods*
  • Electrophoretic Mobility Shift Assay
  • Ethanolamine / chemistry*
  • Genetic Vectors / genetics*
  • Hep G2 Cells
  • Humans
  • Magnetic Resonance Spectroscopy
  • Methacrylates / chemistry*
  • Nylons / chemistry*
  • Plasmids / genetics
  • Polymerization
  • Polymethacrylic Acids / chemistry*
  • Tetrazolium Salts
  • Thiazoles
  • Transfection / methods*
  • beta-Cyclodextrins / chemistry*

Substances

  • Methacrylates
  • Nylons
  • Polymethacrylic Acids
  • Tetrazolium Salts
  • Thiazoles
  • beta-Cyclodextrins
  • poly(2-(dimethylamino)ethyl methacrylate)
  • 2-Hydroxypropyl-beta-cyclodextrin
  • polyglycidyl methacrylate
  • Ethanolamine
  • thiazolyl blue