Effect of umbilical cord serum coated 3D PCL/alginate scaffold for mastoid obliteration

Chul Ho Jang; Yong Beom Cho; Cheol Hee Choi; Yoon Seok Jang; Won-Kyo Jung; Hyeongjin Lee; Geun Hyung Kim

doi:10.1016/j.ijporl.2014.04.004

Effect of umbilical cord serum coated 3D PCL/alginate scaffold for mastoid obliteration

Int J Pediatr Otorhinolaryngol. 2014 Jul;78(7):1061-5. doi: 10.1016/j.ijporl.2014.04.004. Epub 2014 Apr 12.

Authors

Chul Ho Jang¹, Yong Beom Cho², Cheol Hee Choi³, Yoon Seok Jang⁴, Won-Kyo Jung⁵, Hyeongjin Lee⁶, Geun Hyung Kim⁷

Affiliations

¹ Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea; Research Center for Resistant Cells, Chosun University, Gwangju, South Korea. Electronic address: chulsavio@hanmail.net.
² Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea.
³ Research Center for Resistant Cells, Chosun University, Gwangju, South Korea; Department of Bio New Drug Development, Chosun University, Gwangju, South Korea.
⁴ Department of Bio New Drug Development, Chosun University, Gwangju, South Korea.
⁵ Department of Biomedical Engineering, and Center for Marine-integrated Biomedical Technology (BK 21 Plus), Pukyung National University, Busan, South Korea.
⁶ Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
⁷ Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea. Electronic address: xrdghk@gmail.com.

PMID: 24788192
DOI: 10.1016/j.ijporl.2014.04.004

Abstract

Objective: Human umbilical cord serum (hUCS) has a lot of growth factors. To date, there are no reports on stimulating effect of hUCS in osteogenesis. The purpose of this study is to evaluate enhancing effect of hUCS in osteogenesis by mastoid obliteration combined with bony scaffold.

Materials and methods: The fabrication procedure for obtaining PCL/alginate/hUCS was performed. The bulla obliteration was done using PCL/alginate/hUCS in the experimental group and PCL in the control group. To assess the early active mineralization of new bone formation, guinea pigs of each group received an intraperitoneal infusion with alizarin red at 6 weeks post-surgery. The animals of each group were sacrificed 8 weeks post-surgery. Ex vivo microCT and histologic observation were performed.

Results: MicroCT finding shows more radiopaque change within the pores in the experimental group compared to the control group. Stereomicroscopic and SEM findings show new bone formation of the pores in the experimental group. However, the pores between strands almost all remained in the control group. Corresponding histological observations for the stimulatory effects of hUCS showed osteogenesis in the pores between the strands compared to the control group.

Conclusion: Our data suggest that hUCS coated 3D porous PCL scaffold in mastoid obliteration provides enhanced osteogenesis. Therefore, we suggest that our hUCS coated 3D porous PCL could be used in mastoid obliteration in the future.

Keywords: Mastoid obliteration; Osteogenesis; Polycaprolactone; Umbilical cord serum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alginates / chemistry
Animals
Coated Materials, Biocompatible / chemistry
Fetal Blood / chemistry*
Guinea Pigs
Humans
Male
Mastoid / pathology
Mastoid / surgery*
Microscopy, Electron, Scanning
Osteogenesis*
Polyesters / chemistry
Tissue Engineering / methods
Tissue Scaffolds / chemistry*
X-Ray Microtomography

Substances

Alginates
Coated Materials, Biocompatible
Polyesters
polycaprolactone