Abstract
This chapter provides protocols for in vitro and in vivo analysis of TNF-producing cells from a novel TNF reporter mouse. In these transgenic mice, genetic sequence encoding far-red reporter protein Katyushka (FRFPK) was placed under control of the same regulatory elements as TNF, thus providing the basis for detection, isolation, and visualization of TNF-producing cells.
MeSH terms
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Animals
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Encephalomyelitis, Autoimmune, Experimental / metabolism
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Encephalomyelitis, Autoimmune, Experimental / pathology*
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Flow Cytometry
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Genes, Reporter*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism*
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Image Processing, Computer-Assisted
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Indicators and Reagents / metabolism
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism*
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Macrophages / metabolism
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Macrophages / pathology*
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Promoter Regions, Genetic / genetics
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Recombination, Genetic
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Red Fluorescent Protein
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Thy-1 Antigens / genetics
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Tumor Necrosis Factor-alpha / physiology*
Substances
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Indicators and Reagents
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Luminescent Proteins
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Thy-1 Antigens
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Tumor Necrosis Factor-alpha
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enhanced green fluorescent protein
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Green Fluorescent Proteins