Fourier-domain Jones-matrix mapping of a complex degree of mutual anisotropy in differentiation of biological tissues' pathological states

Appl Opt. 2014 Apr 1;53(10):B205-14. doi: 10.1364/AO.53.00B205.

Abstract

This article presents the theoretical background of an azimuthally stable method of Jones-matrix mapping of histological sections of a uterine wall biopsy on the basis of spatial-frequency selection of the mechanisms of linear and circular birefringence. The diagnostic application of a new correlation parameter--a complex degree of mutual anisotropy--is analytically substantiated. The method of measuring coordinate distributions of a complex degree of mutual anisotropy with further spatial filtration of their high- and low-frequency components is developed. The interconnections of such distributions with linear and circular birefringence parameters of the uterine-wall-endometrium histological sections are found. The comparative results of measuring the coordinate distributions of a complex degree of mutual anisotropy formed by fibrillar networks of myosin and collagen fibrils of uterus wall tissue of different pathological states--pre-cancer (dysplasia) and cancer (adenocarcinoma)--are shown. The values and ranges of change of the statistical (moments of the first to fourth orders) parameters of complex degree of mutual-anisotropy coordinate distributions are studied. The objective criteria of diagnosing the pathology and differentiation of its severity degree are determined.

MeSH terms

  • Diagnosis, Computer-Assisted / methods*
  • Diagnosis, Differential
  • Female
  • Fourier Analysis
  • Humans
  • Lasers*
  • Light
  • Refractometry / methods*
  • Reproducibility of Results
  • Scattering, Radiation
  • Sensitivity and Specificity
  • Uterine Neoplasms / diagnosis*
  • Uterine Neoplasms / physiopathology*