The objectives of the studies reported herein were to (1) determine the minimum number of petting simulations required to load the maximum amount of test substance (indoxacarb) residue onto cotton gloves (the sampling medium) from spot-on treated dogs; and (2) using the number of petting simulations that resulted in maximal transfer, to conduct a second study that measured amount of residue dislodged via petting a dog as a function of the time interval after application. Maximal percent transfer of indoxacarb from spot-on treated dogs occurred after 10 repetitive petting simulations (consisting of 3 directional pet strokes each) and was approximately 1-2% of amount applied. Temporal measurements of mean indoxacarb transferability followed an exponential decay function, beginning at approximately 2% transfer on the day of application, and declining to 0.08% by d 30 post treatment.