Comparison of the results of PCR analysis of gene expression in breast cancer tissue specimens stabilized in formalin and RNAlater

Bull Exp Biol Med. 2014 Feb;156(4):486-90. doi: 10.1007/s10517-014-2381-8. Epub 2014 Mar 6.

Abstract

We compared the results of real-time PCR analysis of gene expression in paired specimens breast cancer tissue fixed in RNAlater (Qiagen) stabilization reagent (FF samples) and in formalin (FFPE samples). A clear-cut linear relationship (correlation coefficient 0.76 ± 0.07) was detected between the gene expression logarithm values in FF and FFPE samples. This fact suggests that collections of paraffin blocks with formalin-fixed tissue specimens from patients with a many-year disease history can be effectively used in modern studies.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology
  • Carcinoma, Ductal, Breast / genetics
  • Carcinoma, Ductal, Breast / metabolism*
  • Carcinoma, Ductal, Breast / pathology
  • Female
  • Fixatives / chemistry
  • Formaldehyde / chemistry
  • Gene Expression
  • Gene Expression Profiling / methods*
  • Humans
  • Middle Aged
  • RNA Stability
  • Reagent Kits, Diagnostic
  • Tissue Fixation / methods*

Substances

  • Fixatives
  • Reagent Kits, Diagnostic
  • Formaldehyde