Abstract
A bio-orthogonal click-chemistry procedure was developed to allow the in cell interactome profiling of scalaradial, an anti-inflammatory marine natural product. The results were validated through the application of the classical in vitro chemical proteomics and several bio-physical methods; peroxiredoxins, 14-3-3 isoforms and proteasomes were recognized as main scalaradial targets.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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14-3-3 Proteins / analysis
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14-3-3 Proteins / metabolism*
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Anti-Inflammatory Agents / pharmacology*
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Azides / chemistry
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Click Chemistry / methods*
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HeLa Cells
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Homosteroids / chemistry
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Homosteroids / pharmacology*
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Humans
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Peroxiredoxins / analysis
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Peroxiredoxins / metabolism*
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Sesterterpenes / chemistry
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Sesterterpenes / pharmacology*
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Tandem Mass Spectrometry
Substances
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14-3-3 Proteins
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Anti-Inflammatory Agents
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Azides
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Homosteroids
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Sesterterpenes
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scalaradial
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Peroxiredoxins