In cell scalaradial interactome profiling using a bio-orthogonal clickable probe

Chem Commun (Camb). 2014 Jun 7;50(45):6043-5. doi: 10.1039/c4cc00989d. Epub 2014 Apr 28.

Abstract

A bio-orthogonal click-chemistry procedure was developed to allow the in cell interactome profiling of scalaradial, an anti-inflammatory marine natural product. The results were validated through the application of the classical in vitro chemical proteomics and several bio-physical methods; peroxiredoxins, 14-3-3 isoforms and proteasomes were recognized as main scalaradial targets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins / analysis
  • 14-3-3 Proteins / metabolism*
  • Anti-Inflammatory Agents / pharmacology*
  • Azides / chemistry
  • Click Chemistry / methods*
  • HeLa Cells
  • Homosteroids / chemistry
  • Homosteroids / pharmacology*
  • Humans
  • Peroxiredoxins / analysis
  • Peroxiredoxins / metabolism*
  • Sesterterpenes / chemistry
  • Sesterterpenes / pharmacology*
  • Tandem Mass Spectrometry

Substances

  • 14-3-3 Proteins
  • Anti-Inflammatory Agents
  • Azides
  • Homosteroids
  • Sesterterpenes
  • scalaradial
  • Peroxiredoxins