We report a new and efficient protocol for rapid electrodeposition of a gold-Prussian blue ((Au-PB)REd) nanocomposite with ultrahigh electroactivity on an Au electrode by cyclic voltammetry in 0.1 M aqueous K2SO4 containing 1 mM K3Fe(CN)6, 1 mM HAuCl4, and 0.1 mM Fe2(SO4)3, and an electrochemical quartz crystal microbalance and scanning electron microscopy were utilized to investigate the electrodeposition. The (Au-PB)REd/Au electrode was then cast-coated with a urate oxidase (UOx)-poly(anilineboronic acid) (PABA)-Pt nanoparticle (PtNP) bionanocomposite and chitosan (CS) for high-performance amperometric biosensing of uric acid (UA) in the dual-potential mode. The UOx-PABA-PtNP bionanocomposite was prepared through chemical oxidation of anilineboronic acid (ABA) by sodium chloroplatinate in the presence of UOx. The thus-fabricated CS/UOx-PABA-PtNP/(Au-PB)REd/Au enzyme electrode worked well under optimized conditions through both oxidation and reduction determination of enzyme-generated H2O2, which responded linearly to UA concentrations from 0.3 μM to 0.65 mM with a sensitivity of 223 μA mM(-1) cm(-2) and a limit of detection (LOD) of 0.2 μM (0.7 V vs. SCE), and from 0.2 μM to 0.25 mM with a sensitivity of 247 μA mM(-1) cm(-2) and a LOD of 0.1 μM (-0.05 V vs. SCE), being superior to most analogues hitherto reported for biosensing of UA.