Usefulness of Pneumotest-latex for direct serotyping of Streptococcus pneumoniae isolates in clinical samples

Alicja Kuch; Agnieszka Gołȩbiewska; Izabela Waśko; Patrycja Ronkiewicz; Marlena Markowska; Waleria Hryniewicz; Anna Skoczyńska

doi:10.1128/JCM.00451-14

Usefulness of Pneumotest-latex for direct serotyping of Streptococcus pneumoniae isolates in clinical samples

J Clin Microbiol. 2014 Jul;52(7):2647-9. doi: 10.1128/JCM.00451-14. Epub 2014 Apr 23.

Authors

Alicja Kuch¹, Agnieszka Gołȩbiewska², Izabela Waśko², Patrycja Ronkiewicz², Marlena Markowska², Waleria Hryniewicz², Anna Skoczyńska²

Affiliations

¹ National Reference Centre for Bacterial Meningitis, Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, Poland akuch@cls.edu.pl.
² National Reference Centre for Bacterial Meningitis, Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, Poland.

Abstract

This study evaluated the usefulness of the Pneumotest-Latex assay for serotyping Streptococcus pneumoniae isolates directly in clinical samples. With an agreement of 88.1% with a PCR-based reference method, this test can be a useful tool for this study purpose, especially in clinical laboratories that do not have access to nucleic acid amplification technologies.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques / methods*
Humans
Latex Fixation Tests / methods
Pneumococcal Infections / diagnosis*
Pneumococcal Infections / microbiology*
Serotyping / methods
Streptococcus pneumoniae / classification*
Streptococcus pneumoniae / isolation & purification*