Inkjet printing is a non-contact and low-cost method for high resolution deposition of nanomaterials and mass production of electro-devices. In this study, an environmentally friendly gold nanoparticles (AuNPs) ink was prepared and electrochemical electrodes were fabricated through inkjet printing technology. Electrochemical behaviors of the printed electrodes with different printing layers and laser sintering intensities were studied by cyclic voltammetry. Electrode sintered at 0.6 A with double layers showed a good reproducibility and well linear relation in voltammetric measurement of potassium ferricyanide. Moreover, the prepared electrode was found to be effective for formaldehyde electro-oxidation in alkaline medium. The above results indicated that this AuNPs ink, which can be facilely prepared, may have great potential applications for the preparation of flexible and low-cost electrochemical sensing devices and biosensors by inkjet printing.