Effect of Cbfa1 on osteogenic differentiation of mesenchymal stem cells under hypoxia condition

Zhang-Hua Li; Wen Liao; Qiang Zhao; Tang Huan; Pan Feng; Xia Wei; Yang Yi; Ning-Sheng Shao

Effect of Cbfa1 on osteogenic differentiation of mesenchymal stem cells under hypoxia condition

Int J Clin Exp Med. 2014 Mar 15;7(3):540-8. eCollection 2014.

Authors

Zhang-Hua Li¹, Wen Liao², Qiang Zhao³, Tang Huan¹, Pan Feng¹, Xia Wei³, Yang Yi⁴, Ning-Sheng Shao³

Affiliations

¹ Department of Orthopedics, Renmin Hospital of Wuhan University Wuhan 430060, China.
² Hubei University of Chinese Medicine Wuhan 430065, China.
³ Laboratory of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences Beijing 100850, China.
⁴ Wuhan Sports University Wuhan 430079, China.

PMID: 24753746
PMCID: PMC3992391

Abstract

Objective: To observe the effect of Cbfa1 on biological characteristics of marrow mesenchymal stem cells under hypoxia.

Methods: The second passage of the MSCs were transfected with Cbfa1 and then cultured in 20% O2 and 3% O2 condition individually. The biological features of the cultured MSCs were assessed by the Real-time PCR.

Results: After transfected with Cbfa1, the morphology of MSCs was no significant difference between two oxygen concentrations; The RT-PCR examination revealed that the expression of Cbfa1, BMP, OPN and VEGF in MSCs was higher than those before Cbfa1 transfection (P<0.05), especial the expression of Cbfa1 (P<0.05).

Conclusion: After transfected with Cbfa1, cell morphology or growth cycle of MSCs was not significantly affected, but its osteogenic differentiation potential enhanced, particularly its osteogenic differentiation potential under hypoxia enhanced.

Keywords: Core binding factor alpha l; Mesenchymal stem cell; hypoxia.