Gankyrin is frequently overexpressed in cervical high grade disease and is associated with cervical carcinogenesis and metastasis

PLoS One. 2014 Apr 21;9(4):e95043. doi: 10.1371/journal.pone.0095043. eCollection 2014.

Abstract

Our previous studies have showed that Gankyrin expression is correlated with a malignant phenotype in endometrial carcinoma. Here, we investigated the possible role of Gankyrin in cervical disease. The increasing protein level of Gankyrin was observed in high-grade cervical intraepithelial neoplasia and carcinoma compared with benign cervical tissues and low-grade cervical intraepithelial neoplasia. In para-carcinoma tissues, it was found interestingly that there was no lymph node metastasis when nuclei Gankyrin was positively expressed, but lymph node metastasis rate was 30% (6/20) when nuclei Gankyrin was negatively expressed. In vitro, the transfection of Gankyrin resulted in markedly up-regulating of Vimentin, β-catenin and Twist2, as well as down-regulating of E-cadherin in cervical carcinoma cells. Our results suggested that Gankyrin may be functional in cervical carcinogenesis and metastasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinogenesis / metabolism*
  • Carcinogenesis / pathology*
  • Cell Line, Tumor
  • Cell Proliferation
  • Cervix Uteri / pathology
  • Female
  • Humans
  • Immunohistochemistry
  • Lymphatic Metastasis / pathology*
  • Middle Aged
  • Neoplasm Grading
  • Neoplasm Invasiveness
  • Neoplasm Proteins / metabolism
  • Plasmids / metabolism
  • Proteasome Endopeptidase Complex / metabolism*
  • Proto-Oncogene Proteins / metabolism*
  • RNA, Small Interfering / metabolism
  • Uterine Cervical Neoplasms / metabolism*
  • Uterine Cervical Neoplasms / pathology*

Substances

  • Neoplasm Proteins
  • PSMD10 protein, human
  • Proto-Oncogene Proteins
  • RNA, Small Interfering
  • Proteasome Endopeptidase Complex

Grants and funding

The project was supported by grants from the National Natural Science Foundation of China (NSFC No.81201541), Shanghai Pujiang Talent Program (12PJD002), Science and Research Foundation for Public Welfare Industry of Ministry of Health of China (201002013), Shanghai Science and Technology Committee Foundation(09411962500). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.