Structural investigation of an extracellular polysaccharide produced by the cariogenic bacterium Streptococcus mutans strain UA159

Bo Li; Justyna M Dobruchowska; Michel A Hoogenkamp; Gerrit J Gerwig

doi:10.1016/j.carbpol.2012.05.095

Structural investigation of an extracellular polysaccharide produced by the cariogenic bacterium Streptococcus mutans strain UA159

Carbohydr Polym. 2012 Sep 1;90(1):675-82. doi: 10.1016/j.carbpol.2012.05.095. Epub 2012 Jun 5.

Authors

Bo Li¹, Justyna M Dobruchowska², Michel A Hoogenkamp³, Gerrit J Gerwig²

Affiliations

¹ School of Food Science, Henan Institute of Science and Technology, Hualan Road, 453003 Xinxiang, China.
² Department of Microbiology, University of Groningen, Nijenborgh 7, 8747 AG Groningen, The Netherlands.
³ Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands.

PMID: 24751092
DOI: 10.1016/j.carbpol.2012.05.095

Abstract

The structure of an extracellular polysaccharide EPS159 produced from sucrose by Streptococcus mutans UA159 was investigated through the main oligosaccharides obtained from partial acid hydrolysis, monosaccharide/methylation analysis, and 1D/2D (1)H NMR spectroscopy. The results showed that EPS159 contained terminal, 3-substituted, 6-substituted, and 3,6-disubstituted α-D-glucopyranose residues in a molar percentage of 14, 18, 54, and 14%. The backbone of EPS159 was composed of →6)Glcp(1→ residues, and about 20% of the →6)Glcp(1→ residues was substituted at 3-OH by →3)Glcp(1→ and/or Glcp(1→ residues to form side chains. A composite model of EPS159, that includes all identified structural features, was formulated: [Formula, see text:].

MeSH terms

Cariogenic Agents / chemistry*
Cariogenic Agents / metabolism*
Molecular Structure
Polysaccharides / biosynthesis*
Polysaccharides / chemistry*
Streptococcus mutans / chemistry*
Streptococcus mutans / metabolism*

Substances

Cariogenic Agents
Polysaccharides