The comparison of DPPH-scavenging capacity and anti-inflammatory effects of phenolic compounds isolated from the stems of Stewartia koreana Nakai

Nat Prod Res. 2014;28(17):1409-12. doi: 10.1080/14786419.2014.905560. Epub 2014 Apr 22.

Abstract

The Stewartia koreana Nakai (SK) had been used in oriental traditional medicine as a remedy for acute gastroenteritis, liver diseases, quadriplegia and pain. The antioxidant activity guided isolation 80% methyl extract from stems of SK yielded eight phenolic compounds. We evaluated the anti-oxidative and anti-inflammatory effects of these compounds via assays of 1,1-diphenyl-2-picrylhydazyl (DPPH) radicals and inhibition of nitric oxide (NO) production in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. The results demonstrated that syringaresinol (6) exhibited significant DPPH radical-scavenging activity and inhibitory effects on NO production compared with its positive controls, ascorbic acid and L-NMMA, respectively.

Keywords: Stewartia koreana; antioxidants; nitric oxide; radical scavengers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / isolation & purification*
  • Anti-Inflammatory Agents / pharmacology*
  • Antioxidants / chemistry
  • Antioxidants / isolation & purification*
  • Antioxidants / pharmacology*
  • Ascorbic Acid / pharmacology
  • Biphenyl Compounds / pharmacology*
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / isolation & purification*
  • Free Radical Scavengers / pharmacology*
  • Furans / chemistry
  • Furans / isolation & purification
  • Furans / pharmacology
  • Lignans / chemistry
  • Lignans / isolation & purification
  • Lignans / pharmacology
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects
  • Mice
  • Molecular Structure
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / biosynthesis
  • Oxidation-Reduction
  • Phenols / chemistry
  • Phenols / isolation & purification*
  • Phenols / pharmacology*
  • Picrates / pharmacology*
  • Plant Stems / chemistry
  • Theaceae / chemistry*
  • omega-N-Methylarginine / pharmacology

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Furans
  • Lignans
  • Lipopolysaccharides
  • Phenols
  • Picrates
  • syringaresinol
  • omega-N-Methylarginine
  • Nitric Oxide
  • 1,1-diphenyl-2-picrylhydrazyl
  • Ascorbic Acid