Development of a reverse transcription loop-mediated isothermal amplification assay for the rapid diagnosis of avian influenza A (H7N9) virus infection

J Virol Methods. 2014 Aug:204:101-4. doi: 10.1016/j.jviromet.2014.03.028. Epub 2014 Apr 18.

Abstract

A genetic diagnosis system for detecting avian influenza A (H7N9) virus infection using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) technology was developed. The RT-LAMP assay showed no cross-reactivity with seasonal influenza A (H3N2 and H1N1pdm09) or influenza B viruses circulating in humans or with avian influenza A (H5N1) viruses. The sensitivity of the RT-LAMP assay was 42.47 copies/reaction. Considering the high specificity and sensitivity of the assay for detecting the avian influenza A (H7N9) virus and that the reaction was completed within 30 min, the RT-LAMP assay developed in this study is a promising rapid diagnostic tool for avian influenza A (H7N9) virus infection.

Keywords: Avian influenza virus; H7N9; RT-LAMP; Rapid diagnosis.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Birds
  • Humans
  • Influenza A Virus, H7N9 Subtype / genetics
  • Influenza A Virus, H7N9 Subtype / isolation & purification*
  • Influenza in Birds / diagnosis*
  • Influenza in Birds / virology
  • Influenza, Human / diagnosis*
  • Influenza, Human / virology
  • Nucleic Acid Amplification Techniques / methods*
  • Reverse Transcription*
  • Sensitivity and Specificity
  • Time
  • Zoonoses / diagnosis*
  • Zoonoses / virology