Objective: To clarify the role and mechanism of interleukin (IL)-25 in regulating the biological functions of decidual stromal cells (DSCs) in human early pregnancy.
Design: Laboratory study of the effect of IL-25 induced by hCG on the proliferation of DSCs.
Setting: Research laboratories.
Patient(s): Women aged 23-47 years with normal pregnancy and abortion.
Intervention(s): None.
Main outcome measure(s): Signal transduction from IL-25.
Result(s): Here we show that DSCs coexpress IL-25/IL-17RB. Human chorionic gonadotropin promotes the expression of IL-25/IL-17RB. In contrast to anti-human IL-25 neutralizing antibody, recombinant human IL-25 (rhIL-25) significantly stimulates the proliferation of DSCs in dosage- and time-dependent manners. RhIL-25 promotes the phosphorylation of AKT, extracellular-signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinases (JNK), and nuclear factor κB in DSCs. Interestingly, blocking JNK or AKT signal with inhibitors down-regulates the stimulatory effect on DSC proliferation induced by rhIL-25. In addition, the results of Western blot show that the expression of IL-25/IL-17RB in DSCs from normal pregnancy was higher than that from abortion.
Conclusion(s): Our results have revealed that hCG derived of trophoblasts up-regulates the expression of IL-25/IL-17RB in DSCs and that IL-25 further stimulates the proliferation of DSCs through activating JNK and AKT signals, which finally contributes to the establishment and maintenance of physiological pregnancy.
Keywords: DSCs; IL-17RB; IL-25; early pregnancy; hCG; proliferation.
Copyright © 2014 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.