Crystal structure of FtsA from Staphylococcus aureus

Junso Fujita; Yoko Maeda; Chioko Nagao; Yuko Tsuchiya; Yuma Miyazaki; Mika Hirose; Eiichi Mizohata; Yoshimi Matsumoto; Tsuyoshi Inoue; Kenji Mizuguchi; Hiroyoshi Matsumura

doi:10.1016/j.febslet.2014.04.008

Crystal structure of FtsA from Staphylococcus aureus

FEBS Lett. 2014 May 21;588(10):1879-85. doi: 10.1016/j.febslet.2014.04.008. Epub 2014 Apr 18.

Authors

Affiliations

¹ Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
² National Institute of Biomedical Innovation, 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
³ Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan.
⁴ Laboratory of Microbiology and Infectious Diseases, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
⁵ Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: matsumura@chem.eng.osaka-u.ac.jp.

PMID: 24746687
DOI: 10.1016/j.febslet.2014.04.008

Abstract

The bacterial cell-division protein FtsA anchors FtsZ to the cytoplasmic membrane. But how FtsA and FtsZ interact during membrane division remains obscure. We have solved 2.2 Å resolution crystal structure for FtsA from Staphylococcus aureus. In the crystals, SaFtsA molecules within the dimer units are twisted, in contrast to the straight filament of FtsA from Thermotoga maritima, and the half of S12-S13 hairpin regions are disordered. We confirmed that SaFtsZ and SaFtsA associate in vitro, and found that SaFtsZ GTPase activity is enhanced by interaction with SaFtsA.

Keywords: Bacterial divisome; FtsA; FtsZ; Staphylococcus aureus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / chemistry*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Cell Division
Crystallography, X-Ray
Cytoskeletal Proteins / chemistry
Cytoskeletal Proteins / genetics
Cytoskeletal Proteins / metabolism
Escherichia coli / genetics
GTP Phosphohydrolases / chemistry
GTP Phosphohydrolases / genetics
GTP Phosphohydrolases / metabolism
Models, Molecular
Protein Binding
Protein Multimerization*
Protein Structure, Secondary*
Protein Structure, Tertiary*
Recombinant Proteins / chemistry
Recombinant Proteins / metabolism
Staphylococcus aureus / genetics
Staphylococcus aureus / metabolism*

Substances

Bacterial Proteins
Cytoskeletal Proteins
FtsA protein, Bacteria
FtsZ protein, Bacteria
Recombinant Proteins
GTP Phosphohydrolases