Structure and gene cluster of the O-antigen of Escherichia coli O30

Andrei V Perepelov; Xiyan Hao; Sof'ya N Senchenkova; Alexander S Shashkov; Bin Liu; Lei Wang; Yuriy A Knirel

doi:10.1016/j.carres.2014.02.001

Structure and gene cluster of the O-antigen of Escherichia coli O30

Carbohydr Res. 2014 May 7:389:196-8. doi: 10.1016/j.carres.2014.02.001. Epub 2014 Feb 13.

Authors

Andrei V Perepelov¹, Xiyan Hao², Sof'ya N Senchenkova³, Alexander S Shashkov³, Bin Liu², Lei Wang², Yuriy A Knirel³

Affiliations

¹ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
² TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
³ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.

PMID: 24745322
DOI: 10.1016/j.carres.2014.02.001

Abstract

The acidic O-polysaccharide (O-antigen) of Escherichia coli O30 was isolated from the lipopolysaccharide and studied by sugar analysis and NMR spectroscopy. The following structure of the branched tetrasaccharide repeating unit was established, which is unique among known structures of bacterial polysaccharides: β-D-GlcpNAc--1→2--→4)-β-D-GlcpA-(1→4)-β-D-GlcpA-(1→3)-α-D-GlcpNAc-(1→ The O-antigen gene cluster of E. coli O30 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-polysaccharide structure.

Keywords: Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-Antigen; O-Antigen gene cluster.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Escherichia coli / chemistry*
Escherichia coli / genetics*
Molecular Sequence Data
Multigene Family*
O Antigens / chemistry*
O Antigens / genetics*

Substances

O Antigens