Mucosal adjuvant activity of IL-2 presenting spores of bacillus subtilis in a murine model of Helicobacter pylori vaccination

Krzysztof Hinc; Małgorzata Stasiłojć; Iwona Piątek; Grażyna Peszyńska-Sularz; Rachele Isticato; Ezio Ricca; Michał Obuchowski; Adam Iwanicki

doi:10.1371/journal.pone.0095187

Mucosal adjuvant activity of IL-2 presenting spores of bacillus subtilis in a murine model of Helicobacter pylori vaccination

PLoS One. 2014 Apr 17;9(4):e95187. doi: 10.1371/journal.pone.0095187. eCollection 2014.

Authors

Krzysztof Hinc¹, Małgorzata Stasiłojć², Iwona Piątek², Grażyna Peszyńska-Sularz³, Rachele Isticato⁴, Ezio Ricca⁴, Michał Obuchowski¹, Adam Iwanicki¹

Affiliations

¹ Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland.
² Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdańsk, Gdańsk, Poland.
³ Tri-City Animal Laboratory, Medical University of Gdańsk, Gdańsk, Poland.
⁴ Department of Biology, Federico II University of Naples, Naples, Italy.

Abstract

The endospores of Bacillus subtilis are now widely used as a platform for presentation of heterologous proteins and due to their safety record and high resistance to harsh environmental conditions can be considered as potential vehicles for oral vaccination. In this research we show that recombinant B. subtilis spores presenting a fragment of the Helicobacter acinonychis UreB protein and expressing the ureB gene under vegetative promoter elicit a strong cellular immune response in orally immunized mice when co-administered with spores presenting IL-2. We show for the first time the successful application of two types of recombinant spores, one carrying an antigen and the other an adjuvant, in a single oral immunization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic*
Animals
Bacillus subtilis / physiology*
Bacterial Vaccines / genetics
Bacterial Vaccines / immunology*
Bacterial Vaccines / microbiology
Female
Helicobacter Infections / genetics
Helicobacter Infections / immunology
Helicobacter Infections / metabolism
Helicobacter Infections / prevention & control*
Helicobacter pylori / immunology*
Interleukin-2 / biosynthesis
Interleukin-2 / genetics
Interleukin-2 / immunology*
Mice
Mice, Inbred BALB C
Spores, Bacterial / genetics
Spores, Bacterial / immunology
Spores, Bacterial / metabolism
Vaccination*

Substances

Adjuvants, Immunologic
Bacterial Vaccines
Interleukin-2

Grants and funding

The research was supported by the Polish Ministry of Science and Higher Education on the Support for International Mobility of Scientists Program (project no. 23/MOB/2007/0) and Polish National Science Center grant no. N401 2794 39. Publication costs were covered by the EU FP7 project MOBI4Health, GA. 316094. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.