Abstract
The enterococcal peptide AS-48 exerts a concentration-dependent bactericidal effect on Enterococcus faecalis subsp. liquefaciens S-47; cell rescue by cardiolipin and trypsin can be effected only in the first few minutes after antibiotic addition. Gramicidin-exposed cells are protected from killing by AS-48. Long-term and pulse incorporation of radiolabeled substrates into trichloroacetic acid-precipitable material, O2 consumption, and the ability to maintain intracellular potassium levels are impaired shortly after addition of AS-48.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Anti-Bacterial Agents* / antagonists & inhibitors
-
Anti-Bacterial Agents* / pharmacology*
-
Bacterial Proteins / biosynthesis
-
Cardiolipins / pharmacology
-
DNA, Bacterial / biosynthesis
-
Enterococcus faecalis / drug effects*
-
Enterococcus faecalis / metabolism
-
Gramicidin / pharmacology
-
Leucine / metabolism
-
Microbial Sensitivity Tests
-
Oxygen / metabolism
-
Peptides / antagonists & inhibitors
-
Peptides / pharmacology
-
Potassium / metabolism
-
Sodium / metabolism
-
Trypsin / pharmacology
Substances
-
Anti-Bacterial Agents
-
Bacterial Proteins
-
Cardiolipins
-
DNA, Bacterial
-
Peptides
-
BacA protein, Enterococcus faecalis
-
Gramicidin
-
Sodium
-
Trypsin
-
Leucine
-
Potassium
-
Oxygen