Abstract
Polymyxin B is increasingly used as a treatment of last resort against multidrug-resistant Gram-negative infections. Using a mammalian kidney cell line, we demonstrated that polymyxin B uptake into proximal tubular epithelial cells was saturable and occurred primarily through the apical membrane, suggesting the involvement of transporters in the renal uptake of polymyxin B. Megalin might play a role in the uptake and accumulation of polymyxin B into renal cells.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Anti-Bacterial Agents / metabolism*
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Anti-Bacterial Agents / therapeutic use
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Biological Transport, Active
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Cell Line
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Drug Resistance, Multiple, Bacterial
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Epithelial Cells / cytology
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Epithelial Cells / metabolism*
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Gram-Negative Bacterial Infections / drug therapy
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Gram-Negative Bacterial Infections / microbiology
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Kidney Tubules, Proximal / cytology
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Kidney Tubules, Proximal / metabolism*
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Low Density Lipoprotein Receptor-Related Protein-2 / metabolism*
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Polymyxin B / metabolism*
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Polymyxin B / therapeutic use
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Swine
Substances
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Anti-Bacterial Agents
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Low Density Lipoprotein Receptor-Related Protein-2
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Polymyxin B