A rapid immunomagnetic beads-based immunoassay for the detection of β-casein in bovine milk

F Song; Y Zhou; Y S Li; X M Meng; X Y Meng; J Q Liu; S Y Lu; H L Ren; P Hu; Z S Liu; Y Y Zhang; J H Zhang

doi:10.1016/j.foodchem.2014.02.150

A rapid immunomagnetic beads-based immunoassay for the detection of β-casein in bovine milk

Food Chem. 2014 Sep 1:158:445-8. doi: 10.1016/j.foodchem.2014.02.150. Epub 2014 Mar 12.

Authors

F Song¹, Y Zhou², Y S Li¹, X M Meng³, X Y Meng¹, J Q Liu⁴, S Y Lu¹, H L Ren¹, P Hu¹, Z S Liu¹, Y Y Zhang¹, J H Zhang¹

Affiliations

¹ Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
² Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, PR China. Electronic address: zhouyu69@sina.com.
³ Grain and Oil Food Processing Key Laboratory of Jilin Province, Jilin Business and Technology College, Changchun 130062, PR China.
⁴ Production Quality Test Institute of Jilin Province, Changchun 130022, PR China.

PMID: 24731368
DOI: 10.1016/j.foodchem.2014.02.150

Abstract

An immunomagnetic beads-based enzyme-linked immunosorbent assay (IMBs-ELISA) was developed for the detection of β-casein in bovine milk. Immunomagnetic beads (IMBs) were employed as the solid phase. The anti-β-casein monoclonal antibody (McAb) bound to IMBs was used as capture probe and an anti-β-casein polyclonal antibody (PcAb), labelled with horseradish peroxidase (HRP), was employed as detector probe. Three reaction and two washing steps were needed. Each reaction needed 10 min or less, which significantly shortened detection compared with classic sandwich ELISA. β-Casein in bovine milk was detected across a linear range (2-128 μg mL(-1)). Application results were in accordance with the Kjejdahl method, which suggests the IMBs-ELISA is rapid and reliable for the detection of β-casein in bovine milk.

Keywords: Detection; Enzyme-linked immunosorbent assay; Immunomagnetic beads; Sandwich structure; β-Casein.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Caseins / analysis
Caseins / isolation & purification*
Cattle
Enzyme-Linked Immunosorbent Assay / instrumentation
Enzyme-Linked Immunosorbent Assay / methods*
Milk / chemistry*

Substances

Caseins