Simple assay of trehalose in industrial yeast

Małgorzata Kus-Liśkiewicz; Anna Górka; Mykhailo Gonchar

doi:10.1016/j.foodchem.2014.02.067

Simple assay of trehalose in industrial yeast

Food Chem. 2014 Sep 1:158:335-9. doi: 10.1016/j.foodchem.2014.02.067. Epub 2014 Feb 26.

Authors

Małgorzata Kus-Liśkiewicz¹, Anna Górka², Mykhailo Gonchar³

Affiliations

¹ Biotechnology Centre for Applied and Fundamental Sciences, Department of Biotechnology, Department of Animal Physiology and Breeding, University of Rzeszow, Sokołowska St. 26, 36-100 Kolbuszowa, Poland. Electronic address: mkus@univ.rzeszow.pl.
² Biotechnology Centre for Applied and Fundamental Sciences, Department of Biotechnology, Department of Animal Physiology and Breeding, University of Rzeszow, Sokołowska St. 26, 36-100 Kolbuszowa, Poland.
³ Biotechnology Centre for Applied and Fundamental Sciences, Department of Biotechnology, Department of Animal Physiology and Breeding, University of Rzeszow, Sokołowska St. 26, 36-100 Kolbuszowa, Poland; Institute of Cell Biology, NAS of Ukraine, Drahomanov Street 14/16, Lviv 79005, Ukraine.

PMID: 24731351
DOI: 10.1016/j.foodchem.2014.02.067

Abstract

Trehalose is an essential chemical marker to control a quality of the industrial yeast strains and to assess a tolerance of the yeasts products to different physical stresses. A high-performance liquid chromatography analysis with charged aerosol detection (HPLC-CAD) was developed for trehalose determination in industrial yeasts. The method offers a linearity in the range of 5.0-15 mM with linear regression coefficient R(2)=0.9995, a good reproducibility and relatively short analysis time (7 min). Trehalose can be detected at concentrations as low as 0.07 mM, and limit of precise quantification is 0.2 mM. The coefficient of variation (CV%) is 0.3%. The developed method is more sensitive compared with conventional chromatography procedure with UV absorbance detection. It was shown that the proposed method can be used in baker's industry to control a quality of the yeast products and to assess biotechnological significance of the yeast strains.

Keywords: Baker’s yeast; HPLC with a charged aerosol detection (HPLC–CAD); Trehalose.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid / methods*
Reproducibility of Results
Saccharomyces cerevisiae / chemistry*
Saccharomyces cerevisiae / metabolism
Trehalose / analysis*
Trehalose / metabolism

Substances

Trehalose