Amino group binding peptide aptamers with double disulphide-bridged loops selected by in vitro selection using cDNA display

Chem Commun (Camb). 2014 May 30;50(42):5608-10. doi: 10.1039/c4cc00799a.

Abstract

Peptide aptamers that specifically bind to the amino group on a solid-phase were screened by in vitro selection using the cDNA display method. The identified peptides have a unique structure containing two cyclic loops with disulphide bonds and a linkage region, which were indispensable for molecular recognition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Aptamers, Peptide / chemistry*
  • Aptamers, Peptide / metabolism*
  • DNA, Complementary / genetics
  • Disulfides / chemistry*
  • Magnets / chemistry
  • Microspheres
  • Molecular Sequence Data
  • Peptide Library*
  • Peptides / chemical synthesis*
  • Peptides / chemistry*
  • Peptides / genetics
  • Peptides / metabolism
  • Polystyrenes / chemistry

Substances

  • Aptamers, Peptide
  • DNA, Complementary
  • Disulfides
  • Peptide Library
  • Peptides
  • Polystyrenes