Investigation of a new core-shell particle column for ion-pair reversed-phase liquid chromatography analysis of oligonucleotides

Mirlinda Biba; Christopher J Welch; Joe P Foley

doi:10.1016/j.jpba.2014.03.029

Investigation of a new core-shell particle column for ion-pair reversed-phase liquid chromatography analysis of oligonucleotides

J Pharm Biomed Anal. 2014 Aug 5:96:54-7. doi: 10.1016/j.jpba.2014.03.029. Epub 2014 Mar 26.

Authors

Mirlinda Biba¹, Christopher J Welch², Joe P Foley³

Affiliations

¹ Merck Research Laboratories, Rahway, NJ 07065, USA; Department of Chemistry, Drexel University, Philadelphia, PA 19101, USA. Electronic address: mirlinda_biba@merck.com.
² Merck Research Laboratories, Rahway, NJ 07065, USA.
³ Department of Chemistry, Drexel University, Philadelphia, PA 19101, USA.

PMID: 24727281
DOI: 10.1016/j.jpba.2014.03.029

Abstract

A new core-shell particle column showed excellent performance and durability for separation of short (∼21-mer) ribonucleic acid (RNA) oligonucleotides by ion-pair reversed-phase liquid chromatography (IP-RPLC). Previously investigated core-shell C18 columns showed excellent peak shapes and separations of closely eluting impurities by IP-RPLC. However, these columns showed only modest long-term stability at the neutral pH and elevated column temperatures of ≥60°C, typically used for IP-RPLC analysis of oligonucleotides. The newly introduced SunShell C18 column provided separations comparable to the previously evaluated core-shell columns, but with significantly improved long-term column stability when operated at neutral pH and elevated column temperature.

Keywords: Column stability; Core–shell particle column; High temperature RPLC; Ion-pair reversed-phase liquid chromatography; Oligonucleotides.

Publication types

Comparative Study

MeSH terms

Chromatography, Reverse-Phase / methods*
Hydrogen-Ion Concentration
Oligonucleotides / analysis*
Oligonucleotides / chemistry
Temperature
Time Factors

Substances

Oligonucleotides