Label-free dendrimer-like silica nanohybrids for traceable and controlled gene delivery

Biomaterials. 2014 Jul;35(21):5580-90. doi: 10.1016/j.biomaterials.2014.03.051. Epub 2014 Apr 13.

Abstract

To create advanced functional nanocarriers for achieving excellent gene delivery performance, fluorescence label-free hybridized dendrimer-like silica nanocarriers (HPSNs-AC-PEI) were developed by using the endosomal pH and cytoplasmic glutathione (GSH) responsive autofluorescent acetaldehyde-modified-cystine (AC) to link non-toxic low molecular weight branched polyethyleneimine (PEI) onto amino-functionalized dendrimer-like silica nanoparticles with hierarchical pores (HPSNs-NH2). The specific microstructure of this hybridized nanocarrier makes it not only show low cytotoxicity and high gene loading capability, but also display high gene transfection efficiency. The cleavage of disulfide bonds caused by GSH facilitates plasmid DNA (pDNA) release. Moreover, the pH and GSH controlled gene delivery profile can be real-time tracked using the autofluorescence of HPSNs-AC-PEI.

Keywords: Dendrimer-like; Fluorescence; GSH; Gene delivery; Mesoporous silica; Responsive.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Cricetulus
  • DNA / chemistry
  • Dendrimers / chemistry*
  • Drug Carriers / chemistry
  • Drug Delivery Systems / methods
  • Gene Transfer Techniques
  • Glutathione / metabolism
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Microscopy, Confocal
  • Microscopy, Electron, Scanning
  • Molecular Weight
  • Nanoparticles / chemistry
  • Plasmids / genetics
  • Polyethyleneimine / chemistry
  • Silicon Dioxide / chemistry*
  • Transfection

Substances

  • Dendrimers
  • Drug Carriers
  • Green Fluorescent Proteins
  • Silicon Dioxide
  • Polyethyleneimine
  • DNA
  • Glutathione