Bioassay guided isolation and identification of anti-Acanthamoeba compounds from Tunisian olive leaf extracts

Ines Sifaoui; Atteneri López-Arencibia; Juan Carlos Ticona; Carmen M Martín-Navarro; María Reyes-Batlle; Mondher Mejri; Jacob Lorenzo-Morales; Antonio Ignacio Jiménez; Basilio Valladares; Isabel Lopez-Bazzocchi; Manef Abderabba; José E Piñero

doi:10.1016/j.exppara.2014.02.018

Bioassay guided isolation and identification of anti-Acanthamoeba compounds from Tunisian olive leaf extracts

Exp Parasitol. 2014 Nov:145 Suppl:S111-4. doi: 10.1016/j.exppara.2014.02.018. Epub 2014 Apr 12.

Affiliations

¹ Laboratoire Matériaux-Molécules et Applications, IPEST, B.P. 51, 2070 La Marsa, University of Carthage, Tunisia. Electronic address: ines.sifaoui@hotmail.com.
² University Institute of Tropical Diseases and Public Health, University of La Laguna, Avda Francisco Sanchez s/n, Campus de Anchieta, 38271 la Laguna, Tenerife, Canary Islands, Spain.
³ Instituto Universitario de Bio-Orgánica Antonio González, Universidad de La Laguna, Tenerife, Canary Islands, Spain.
⁴ University Institute of Tropical Diseases and Public Health, University of La Laguna, Avda Francisco Sanchez s/n, Campus de Anchieta, 38271 la Laguna, Tenerife, Canary Islands, Spain; Centre for Integrative Physiology, School of Biomedical Sciences, University of Edinburgh, Edinburgh, Scotland, United Kingdom.
⁵ Laboratoire Matériaux-Molécules et Applications, IPEST, B.P. 51, 2070 La Marsa, University of Carthage, Tunisia.

PMID: 24726697
DOI: 10.1016/j.exppara.2014.02.018

Abstract

Pathogenic Acanthamoeba strains are causative agents of Granulomatous Amoebic Encephalitis (GAE) and Acanthamoeba keratitis (AK) worldwide. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. The aim of this study was to investigate the activity of Limouni olive leaf cultivar against the trophozoite stage of Acanthamoeba. The ethyl acetate and methanol extracts of this variety were tested against Acanthamoeba castellanii Neff. The ethyl acetate extract of olive leaf was the most active showing an IC50 of 5.11±0.71μg/ml of dry extract. Bio-guided fractionation of this extract was conducted and led to the identification of three active compounds namely oleanolic and maslinic acids and oleuropein which could be used for the development of novel therapeutic approaches against Acanthamoeba infections.

Keywords: Acanthamoeba; Bioassay fractionation; Chemotherapy; Olive leaf extract.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acanthamoeba castellanii / drug effects*
Acanthamoeba castellanii / growth & development
Biological Assay
Chromatography, Gel
Inhibitory Concentration 50
Olea / chemistry*
Parasitic Sensitivity Tests
Plant Extracts / isolation & purification
Plant Extracts / pharmacology*
Plant Leaves / chemistry
Trophozoites / drug effects
Trophozoites / growth & development
Tunisia

Substances

Plant Extracts