First isolation and molecular characterization of foot-and-mouth disease virus in Benin

Vet Microbiol. 2014 Jun 25;171(1-2):175-81. doi: 10.1016/j.vetmic.2014.03.003. Epub 2014 Mar 12.

Abstract

Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. It is one of the most economically devastating diseases affecting livestock animals. In West Africa, where constant circulation of FMD virus (FMDV) is assumed, very few studies on the characterization of circulating strains have been published. This study describes the first isolation and characterization of FMDV in Benin. FMDV was isolated from 42 samples. Antigen Capture Elisa (Ag-ELISA) and VP1 coding sequence analysis revealed 33 strains of serotype O and 9 strains of serotype A. Phylogenetic analysis of the VP1 sequence revealed two different groups of type O isolates and one group of A isolates. VP1 sequence comparison with the sequences available in the GenBank database revealed a close relationship of the Benin isolates with topotype O of West Africa and with African topotype A of genotype VI. Knowledge of the recent strains circulating in Benin should contribute to better selection of vaccine strains and enable the updating of molecular epidemiology data available for West Africa in general.

Keywords: Benin; Foot-and-mouth disease; Virus isolation; West Africa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Africa, Western
  • Amino Acid Sequence
  • Animals
  • Benin
  • Capsid Proteins / genetics
  • Cattle
  • Cattle Diseases / virology*
  • Foot-and-Mouth Disease / virology*
  • Foot-and-Mouth Disease Virus / classification
  • Foot-and-Mouth Disease Virus / genetics*
  • Foot-and-Mouth Disease Virus / isolation & purification*
  • Genotype
  • Molecular Sequence Data
  • Phylogeny
  • Serotyping

Substances

  • Capsid Proteins
  • VP1 protein, Foot-and-mouth disease virus