Improving oral human papillomavirus detection using toothbrush sampling in HIV-positive men who have sex with men

Jason J Ong; Tim Read; Marcus Chen; Sandra Walker; Matthew Law; Catriona Bradshaw; Suzanne M Garland; Sepehr N Tabrizi; Alyssa Cornall; Andrew Grulich; Jane Hocking; Christopher K Fairley

doi:10.1128/JCM.00286-14

Improving oral human papillomavirus detection using toothbrush sampling in HIV-positive men who have sex with men

J Clin Microbiol. 2014 Jun;52(6):2206-9. doi: 10.1128/JCM.00286-14. Epub 2014 Apr 9.

Authors

Affiliations

¹ Melbourne School of Population and Global Health, University of Melbourne, Melbourne, Australia j.ong@unimelb.edu.au.
² Melbourne Sexual Health Centre, Melbourne, Australia Central Clinical School, Monash University, Clayton, Australia.
³ Melbourne Sexual Health Centre, Melbourne, Australia.
⁴ Kirby Institute, University of New South Wales, Sydney, Australia.
⁵ Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Australia Regional HPV Lab Net Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia Murdoch Childrens Research Institute, Parkville, Australia.
⁶ Regional HPV Lab Net Reference Laboratory, Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia.
⁷ Melbourne School of Population and Global Health, University of Melbourne, Melbourne, Australia.

Abstract

Pre- and postabrasion oral rinse samples (ORS) and a toothbrush sample detected human papillomavirus (HPV) DNA in at least one sample among 45 (26%) of 173 HIV-positive men who have sex with men. There was moderate agreement for HPV genotype detection between the preabrasion and postabrasion ORS (κ = 0.49; 95% confidence interval [CI], 0.37 to 0.61). There was good agreement between postabrasion ORS and toothbrushes (κ = 0.70; 95% CI, 0.60 to 0.80). The sensitivities for HPV genotypes detected were 80% (95% CI, 69 to 88) for preabrasion ORS, 65% (95% CI, 54 to 76) for postabrasion ORS, and 75% (95% CI, 63 to 84) for toothbrushes.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Aged, 80 and over
Diagnosis, Oral / methods*
HIV Infections / complications
Homosexuality, Male
Humans
Male
Middle Aged
Papillomaviridae / isolation & purification*
Papillomavirus Infections / diagnosis*
Sensitivity and Specificity
Specimen Handling / methods*
Young Adult