Antioxidative Flavonol Glucuronides and Anti-HBsAg Flavonol from Rotala rotundifolia

Li-Jie Zhang; Sheau-Farn Yeh; Ya-Ten Yu; Li-Ming Yang Kuo; Yao-Haur Kuo

doi:10.1016/s2225-4110(16)30057-8

Antioxidative Flavonol Glucuronides and Anti-HBsAg Flavonol from Rotala rotundifolia

J Tradit Complement Med. 2011 Oct;1(1):57-63. doi: 10.1016/s2225-4110(16)30057-8.

Authors

Li-Jie Zhang¹, Sheau-Farn Yeh², Ya-Ten Yu¹, Li-Ming Yang Kuo³, Yao-Haur Kuo⁴

Affiliations

¹ National Research Institute of Chinese Medicine, Taipei 112, Taiwan.
² Institute of Biochemistry and Molecular Biology, National Yang-ming University, Taipei, 112, Taiwan.
³ School of Pharmacy, Taipei Medical University, Taipei, 110, Taiwan.
⁴ National Research Institute of Chinese Medicine, Taipei 112, Taiwan ; The Graduate Institute of Integrated Medicine, China Medical University, Taichung, 404, Taiwan.

Abstract

Two new flavonol glucuronides, quercetin 3-O-β-D-2″-acetylglucuronide (1) and quercetin 3-O-β-D-2″-acetylglucuronide methyl ester (2), along with four known flavonoids (3-6) were isolated from the whole parts of Rotala rotundifolia. The structures of 1 and 2 were elucidated by application of various spectroscopic methods, including 1D and 2D NMR techniques. Biological evaluation showed that all of isolated flavonoid compounds have potent anti-oxidative activities by DPPH and superoxide anion methods, and kaempferol (3) and quercetin (4) exhibited significant anti-HBV activity assayed by anti-HBsAg production. The HPLC fingerprint with photodiode array detection (HPLC-DAD) for quality control of R. rotundifolia partitioned EtOAc layer was also established.

Keywords: Anti-HBsAg; Anti-oxidative activity; Flavonol glucuronides; Rotala rotundifolia.