The purpose of this study was to investigate the effect of performance jewelry embedded with both a hologram and negative ions on maximal oxygen uptake (V[Combining Dot Above]O2max), V[Combining Dot Above]O2 at ventilatory threshold (V[Combining Dot Above]O2VT), or heart rate at ventilatory threshold (HRVT) measured through metabolic gas exchange. Eight males and 10 females (age = 26.5 ± 7.18 years) volunteered to participate in this study. Silicone bracelets with both hologram technology and negative ions were used as the "programmed" device. An identical silicone bracelet that did not contain the negative ion or hologram technology was used as the "nonprogrammed" device. A third condition in which the participants did not wear a band served as a control trial. The type of band worn during testing sessions was blinded from the participants and researchers. The order of testing was randomized. Subjects underwent 3 maximal graded exercise tests separated by 1 week. A repeated measure analysis of variance was used to test for mean differences across the 3 trials. The mean V[Combining Dot Above]O2max when wearing the programmed band (52.32 ± 4.79 ml·kg·min), when wearing the nonprogrammed band (51.47 ± 5.89), and without a band (53.32 ± 7.63 ml·kg·min) were found to be of no significant difference (p = 0.494). Assessment of the subjects HRVT under the 3 conditions (programmed, nonprogrammed, and no band) yielded no significant difference (p = 0.633). The HRVT of the above-mentioned 3 conditions were 136.06 ± 16.33, 135.00 ± 19.48, and 130.41 ± 24.39, respectively. The V[Combining Dot Above]O2VT for the programmed band (30.04 ± 6.91 ml·kg·min), nonprogrammed (30.77 ± 8.125 ml·kg·min), and no band (27.27 ± 8.66 ml·kg·min) were not significantly different (p = 0.221). The results of this study provide data that holographic and negative ion technology wristbands may have no effect on V[Combining Dot Above]O2max, V[Combining Dot Above]O2 at ventilatory threshold, or HRVT, and thus may have no positive effect on exercise endurance.