Optimized nested polymerase chain reaction for antemortem detection of Mycobacteria in Amazon parrots (Amazona aestiva) and orange-winged Amazons (Amazona amazonica)

J Zoo Wildl Med. 2014 Mar;45(1):161-4. doi: 10.1638/2013-0019R1.1.

Abstract

The objectives of this study were to optimize nested polymerase chain reaction (PCR) for Mycobacterium avium complex and Mycobacterium tuberculosis complex and apply them on samples from parrots. Results were negative for the presence of these Mycobacterium in the samples, and nested PCR was specific, faster, and more sensitive than other tests, thereby justifying its use in antemortem diagnosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amazona*
  • Animals
  • Bird Diseases / diagnosis
  • Bird Diseases / microbiology*
  • Mycobacterium / isolation & purification*
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / veterinary*
  • Sensitivity and Specificity